Journal Club Questions

• Biological motivation?
• Experimental design
•  How were cells grown, what genetic background(s)?
•  Choice of reference(s)?
•  How many biological / technical replicates?
• Array technology used – was this the best available technology?
•  Are there better technologies today?
• Experimental Pitfalls
• What could cause misleading or artifactual expression
• Computational Analyses
• Normalization
• How were results filtered / grouped / organized  to establish relationships or networks?
• Summary of novel results / major findings
• Any new technologies / methods developed?
• Alternative Methods for Verification of major findings
• What are the major forms of data presentation?  Are these effective for illustrating complex / large data sets?
  
• What kinds of follow-up experiments were done?
• Wet-lab
• Computational
• Can you think of other follow-up experiments, either using microarrays, traditional molecular biology, or computational analyses?