This course will explore both theoretical and practical concepts of bioinstrumentation and its application in a variety of medical and scientific disciplines. Analytical experiments will be performed in the laboratory portion of the class. Topics covered include plasmid purification from bacteria, transformation of plasmid DNA to bacteria, Polymerase Chain Reaction (PCR), DNA sequencing with Pyrosequencing, recombinant protein production, protein purification with affinity chromatography and gel-electrophoresis of DNA and protein. This course highlights the basic laboratory skills that are essential for beginning-level employment in clinical, pharmaceutical and biotechnology laboratories. This course is well-suited for students in all majors and programs. In addition, the course involves a lab demo as well as literature workshop of selected articles, which will be performed in groups. Each group presents one article and will oppose two other groupsÂ’ articles. This project aims to teach critical reading, interpretation and comparison of the most advanced techniques and platforms in the fields of genomics proteomics and related nstrumentations. The project requires teamwork and planning, and participation as well as presence on the laboratory demos as well workshop days is compulsory.
Professor Nader Pourmand
pourmand@soe.ucsc.edu
Teaching Assistant:
John Kim
hyjkim@soe.ucsc.edu
Office hours are available by appointment.